The Annals of Applied Statistics

Regression analysis for microbiome compositional data

Pixu Shi, Anru Zhang, and Hongzhe Li

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Abstract

One important problem in microbiome analysis is to identify the bacterial taxa that are associated with a response, where the microbiome data are summarized as the composition of the bacterial taxa at different taxonomic levels. This paper considers regression analysis with such compositional data as covariates. In order to satisfy the subcompositional coherence of the results, linear models with a set of linear constraints on the regression coefficients are introduced. Such models allow regression analysis for subcompositions and include the log-contrast model for compositional covariates as a special case. A penalized estimation procedure for estimating the regression coefficients and for selecting variables under the linear constraints is developed. A method is also proposed to obtain debiased estimates of the regression coefficients that are asymptotically unbiased and have a joint asymptotic multivariate normal distribution. This provides valid confidence intervals of the regression coefficients and can be used to obtain the $p$-values. Simulation results show the validity of the confidence intervals and smaller variances of the debiased estimates when the linear constraints are imposed. The proposed methods are applied to a gut microbiome data set and identify four bacterial genera that are associated with the body mass index after adjusting for the total fat and caloric intakes.

Article information

Source
Ann. Appl. Stat. Volume 10, Number 2 (2016), 1019-1040.

Dates
Received: June 2015
Revised: January 2016
First available in Project Euclid: 22 July 2016

Permanent link to this document
http://projecteuclid.org/euclid.aoas/1469199903

Digital Object Identifier
doi:10.1214/16-AOAS928

Mathematical Reviews number (MathSciNet)
MR3528370

Zentralblatt MATH identifier
06625679

Keywords
Compositional coherence coordinate descent method of multipliers high dimension log-contrast model model selection regularization

Citation

Shi, Pixu; Zhang, Anru; Li, Hongzhe. Regression analysis for microbiome compositional data. Ann. Appl. Stat. 10 (2016), no. 2, 1019--1040. doi:10.1214/16-AOAS928. http://projecteuclid.org/euclid.aoas/1469199903.


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Supplemental materials

  • Supplement to “Regression analysis for microbiome compositional data”. The online Supplemental Materials include proofs of all lemmas and theorems [Shi, Zhang and Hongzhe (2016)].